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Research Laboratory Technician
San Antonio, TX 

This is a DoD research effort forcing on applied translational research aimed at reducing mortality and morbidity of severe combat casualty. Traumatic hemorrhage (TH) represents the leading cause of death from severe traumatic injuries in both military and civilian environments. TH accounted for 45-68% of U.S. military deaths in Operation Iraqi Freedom, and 90% of these deaths occurred prior to hospital arrival. Recent clinical and experimental studies have brought increasing evidence for activation of the innate immune system in contributing to the pathogenesis and adverse outcomes.

Our program is primary goal is to identify using animal models of TH and supporting clinical studies, selected immune-inflammatory molecules of therapeutic value to be administered early after injury in a pre-hospital setting. This work requires an expert understanding of molecular biology, protein biochemistry, immunology and medicine. Our Team member have experience in the field of medicine with educational degrees of Ph.D, M.D., M.S., B.S. or extensive experience in their particular technical areas of expertise.

This position will be performed within USAISR’s Prolonged Field Care (PFC) Task Area.  The candidate will provide research activity support services to include task area support, and protocol support.  More specifically, the positions will support the USAISR in areas that require tracking of Assist in the execution of certain protocols to include data collection, maintaining documentation, regulatory binders, and analysis of data. Perform micro-surgery on small animals such as rodents, insert vascular catheters, implantable transmitters, and assist in surgical procedures on large animal models such as Swine.



  • Assist and/or perform acute and recovery surgeries in small and large animals using sterile techniques
  • Collect physiological data (e.g. blood pressure, heart rate, muscle strength)
  • Perform cut downs for the placement of arterial and venous catheters in small and large animals
  • Set-up, maintain and calibrate electronic physiological devices with PC based data acquisition and control systems
  • Provide daily assessments on small and large animals and administer analgesics
  • Euthanize animals, dissect, and harvest tissues and process them for histology, specified bioassays, and storage
  • Perform routine molecular biology assays (e.g., RT-PCR, Western Blot)
  • Perform microscopic analysis using multiple microscopy techniques such as, but not limited to fluorescence and confocal microscopy
  • Maintain and organize digital archive of photomicrographs
  • Perform general microbiological and cell culture methods
  • Performs data collection, management, and analysis for preparation for publication
  • Maintains laboratory notebooks
  • Track and maintain inventory of tissue samples
  • Maintains organization of laboratory areas, and maintains and order all pertinent supplies related to protocols. 
  • Assists in submitting manuscripts, posters and abstracts to relevant journals and meetings. 
  • Order and provide animal handling of small and large animals including surgical preparation



  • Bachelor’s or Master’s degree in science related field
  • Handing animals in a BSL2 environment.
  • Must hold ALACC accreditation for working with laboratory animals.
  • A minimum of 1 years of experience in conducting small animal surgeries.
  • Demonstrated hands on experience working with complement system modulation/inhibition using biological animal models and performing lab assays to determine complement activity/deposition in tissues/organs.
  • Demonstrated experience with sterile surgical procedures on large animals.
  • Experience in large animal models of traumatic hemorrhage.
  • Operational knowledge of advanced lab equipment; familiarity with computing equipment to include physiological data acquisition and analysis.
  • Ability to work independently as well as with a team.
  •  Must be a US citizen



  • Must have skill and the ability to follows established research protocols
  • Be knowledgeable of and ability to enrolled in annual required safety training classes.
  • Must have skill in collects blood samples and determines plasma/serum metabolic changes Assists senior scientists to determine histological changes in tissues.
  • Skills and ability of collects tissue samples extracted during animal necropsy and prepare them for analysis.
  • Skilled in prepares in post-experiment processing of animal specimens such as frozen tissue blocks for frozen section cut.
  • Experience in proper documentation of surgical procedures and IACUC mandated regulatory materials 
  • Skills in assisting with instrumentation, monitoring, surgical preparation, care and observation during recovery, administration of experimental treatments, sample collection, and observation associated with the collection of research data surgical procedures
  • Ability to do daily calibration of monitoring equipment and analog-to-digital data collection system.

Work Environment Will be performance duties within a 100 % secured military controlled installation, the duties of this position are primarily performed in a climate controlled office environment. Occasional travel and overnight stay may be required. Will requires long periods of Standing, working at a desk, and computer terminals. Will travel between military bases as needed.


This program performs services and support compatible with the medical facilities operating capacity and equipment to help organize and oversee a global Air Force Medical Services wide, medical research, clinical and translational, investigative research activities and resources. Support is to provide patient-oriented research program directed to the mechanisms of traumatic hemorrhage and the related problems associated with addressing therapeutic interventions through translational large and small animal research methods. The translational research program addresses a need to develop new clinical practice guidelines, technologies related to combat casualty care in military medicine to mitigate outcomes from hemorrhagic associated injuries. The program scope is limited to applied clinical knowledge and medical technologies to deliver solutions addressing the effects from combat related injuries involving hemorrhage. Translational medicine projects is designed to directly connect research to patient care to include development and application of new technologies in a patient driven environment with an emphasis on early patient evaluation and treatment. Clinical Research and Engineering (CR&E) services shall encompass providing a holistic approach to clinical research to address knowledge gaps, applied medical technology development and translational medicine that will expedite adoption into clinical practice from bench to the bedside.

Applicants selected will be subject to a U.S. Government security investigation and must meet eligibility requirements for access to classified information.

Cherokee Nation Technology Solutions provides technical support services and project support personnel to our Government and industry partners. Our highly skilled experts support and supplement the mission, expertise, and skill sets of our clients. Utilizing our customer-intimate approach, we partner with our clients to provide strategic solutions that maximize the use of human and financial resources to ensure that mission-critical projects are completed on time and within budget. 

We are an equal opportunity/affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, protected Veteran status, gender identity and sexual orientation. If you’d like more information about your EEO rights as an applicant under the law, please visit the following two sites:

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